The proteomics facility, headed by Mariette Matondo, is part of the Mass Spectrometry for Biology Utechs (MSBio) directed by Julia Chamot-Rooke. The overall objective of the platform is to develop innovative bottom-up proteomics strategies to address protein function, regulation and dynamic interactions, in particular in the context of infectious diseases. Our collaborators benefit from the expertise of the staff, as well as state-of-the-art instrumentation, including the latest generation of high speed, high-resolution mass spectrometers (Orbitrap), and bioinformatics tools. The proteomics analyses provided by the platform include:
• Identification of low abundant proteins in complex biological matrices
• Advanced quantitative proteomic strategies
• Characterization of post-translational modifications (PTMs)
• Analysis and dynamics of protein complexes
• Top-Down Proteomics to identify proteoforms.
• Coming soon : Single cell proteomics
Analyses can be performed either as research collaboration or service. Prior to starting the project, an experimental design is defined, and the staff provides advice on sample preparation. Results are further provided after a bioinformatic analysis of the data.