Cytotoxicity Bioassay Platform

Fiocruz’s Network Technological Platforms

The platform performs cytotoxicity bioassays to determine IC50 values and predict LD50 values. It also offers the contact cytotoxicity assay that allows a direct toxicity assessment of the test item on the test system (cells). The colorimetric assays (neutral red dye uptake and MTT assay) use the multi-user equipment Synergy H1M2F microplate reader, also available for autonomous use and training (exclusive for internal users) or use with the platform staff. In addition, other medium and small equipment is also used, such as class IIB2 biological safety booths, monochannel and multichannel micropipettes, analytical balance, CO2 incubators, inverted microscope with photographic system.

This platform is located in FIOCRUZ – Paraná.