P3M : Proteomics and Modified Proteins analysis Platform

PAGés-P3M is the glycomic and proteomic plateforme of the UAR (Unité d’Appui et de Recherche) PLBS (Plateformes Lilloises en Biologie & Santé) UMS2014 US41. This plateforme is composed of 2 laboratories, one team (PAGés) at the Cité scientifique and one team (P3M) at the Institute Pasteur de Lille.

P3M offers expertise ranging from the identification of proteomes by nanoLC-MSMS and the use of databases or de novo analysis, to their quantification with or without labeling.

The plateforme develops Thermal Proteome Profiling (TPP) analysis for the identification of interactomes involved in the action of drugs, metabolic pathways, or even modulation of gene expression.

The available equipment also allows the study of post-translational modifications (PTM) of small sizes with or without enrichment, as well as the characterization of protein maturation events.

P3M collaborates with PAGés platform for the specific study of glycosylations at the scale of sugars, glycopeptides and glycoproteins.

The proteomics facility, headed by Mariette Matondo, is part of the Mass Spectrometry for Biology Utechs (MSBio) directed by Julia Chamot-Rooke. The overall objective of the platform is to develop innovative bottom-up proteomics strategies to address protein function, regulation and dynamic interactions, in particular in the context of infectious diseases. Our collaborators benefit from the expertise of the staff, as well as state-of-the-art instrumentation, including the latest generation of high speed, high-resolution mass spectrometers (Orbitrap), and bioinformatics tools. The proteomics analyses provided by the platform include:
• Identification of low abundant proteins in complex biological matrices
• Advanced quantitative proteomic strategies
• Characterization of post-translational modifications (PTMs)
• Analysis and dynamics of protein complexes
• Top-Down Proteomics to identify proteoforms.
• Coming soon : Single cell proteomics
Analyses can be performed either as research collaboration or service. Prior to starting the project, an experimental design is defined, and the staff provides advice on sample preparation. Results are further provided after a bioinformatic analysis of the data.

The main objective of the platform is to provide researchers with untargeted metabolomic strategies to help them answer their biological questions. The metabolomic team support researchers from the definition of experimental design to the interpretation of the results. They benefit from state-of-the-art instrumentation and computational methods. It includes the latest generation of high speed, high-resolution mass spectrometers as well as bioinformatics and statistical tools to analyze the complex high-dimensional data generated.
Our team is composed of experts in Mass Spectrometry (MS), metabolomics, lipidomics, computational and Mass Spectrometry Imaging (MSI)