Fiocruz Network Facilities

The Electron Microscopy Facility gives support at sample preparation, training on proper use of instruments related to sample preparation and image acquisition. The FEI Tecnai G2 Spirit Biotwin Transmission Electron Microscope has a resolution of 0.34 nm and operates at a voltage of 20 kV to 120 kV, with options for tungsten (W) or lanthanum hexaboride (LaB6) filament and magnification of up to 300,000x. Equipped with MegaView Olympus 2K and Eagle 4K CCD cameras, as well as FEI’s Tecnai Imaging & Analysis (TIA) image capture software. Enabled for analyzing the ultrastructure of samples in 2D. Also, the JSM-5600LV Scanning Electron Microscope is a conventional high and low vacuum microscope with tungsten (W) filament and equipped with secondary and backscattered electron detectors for topographic analysis of samples. The microscope has a resolution of 5 nm and an acceleration voltage of 0.5-30 kV.

This facility is located at FIOCRUZ – Pernambuco.

Fiocruz Network Facilities

The Fluorescence Microscopy Facility offers image acquisitions on Leica DMi8. This microscope has 5, 10, 20, 40, 63 and 100X objectives with DIC. Fluorescence module with filters and cubes for DAPI, FITC, RHOD and Y5. Stage with adjustable universal support for slides up to 120 mm long and petri dishes with diameters between 20 and 68 mm and support for bottles and culture plates. High sensitivity monochromatic camera for fluorescence and colored camera for bright field modalities. Software for image acquisition and analysis with deconvolution.

This facility is located at FIOCRUZ – Pernambuco.