ARIADNE
ARIADNE is composed of two platforms: ARIADNE-Criblage, High-Content and High-throughput-Screening Platform and ARIADNE-ADME.
Working independently but complementary, their objective is to discover small chemical molecules useful in therapy or to explore the living, and to characterize their ADME properties. ARIADNE-Criblage brings together a set of expertise and equipment dedicated to the screening of biological disruptors such as small chemical molecules or siRNA libraries. ARIADNE-ADME allows to quantify the parameters which characterize the ability of molecules to be Absorbed, Distributed, Metabolized and Eliminated in vivo (ADME). Evaluation of ADME parameters early in the discovery process is widely used during target validation, hit-discovery, hit-to-lead optimisation stages
The proteomics facility, headed by Mariette Matondo, is part of the Mass Spectrometry for Biology Utechs (MSBio) directed by Julia Chamot-Rooke. The overall objective of the platform is to develop innovative bottom-up proteomics strategies to address protein function, regulation and dynamic interactions, in particular in the context of infectious diseases. Our collaborators benefit from the expertise of the staff, as well as state-of-the-art instrumentation, including the latest generation of high speed, high-resolution mass spectrometers (Orbitrap), and bioinformatics tools. The proteomics analyses provided by the platform include:
• Identification of low abundant proteins in complex biological matrices
• Advanced quantitative proteomic strategies
• Characterization of post-translational modifications (PTMs)
• Analysis and dynamics of protein complexes
• Top-Down Proteomics to identify proteoforms.
• Coming soon : Single cell proteomics
Analyses can be performed either as research collaboration or service. Prior to starting the project, an experimental design is defined, and the staff provides advice on sample preparation. Results are further provided after a bioinformatic analysis of the data.
The nanoimaging core facility focusses on providing high-end cryo-electron microscopy services. We specialize in the three major workflows: single particle analysis (SPA), cryo-electron tomography/cryo focused ion-beam milling and correlative light and electron microscopy (cryo-FIB/cryo-CLEM) and microcrystal electron diffraction (MED). We help users collect their data but also organize many courses to train users to become fully autonomous. Our equipment is state-off-the art fully upgraded to the latest Thermofisher standards.
The collection of Cyanobacteria maintains the collection Pasteur Cultures of Cyanobacteria (PCC), 800 monoclonal pure strains of Cyanobacteria.
This platform is part of the Photonic BioImaging Unit of Technology and Service (UTechS) of the Institut Pasteur
The mission of the PFBMI is to provide an added value to a great variety of biological projects, enabling the characterization of the intrinsic properties of macromolecules and their assemblies (size, shape, folding, stability auto-association,…) and of the interactions in which they are involved (stoichiometry, thermodynamic and kinetic parameters, …). PFBMI equipments are available for the entire scientific community, in France and abroad, but a priority is given to teams from Institut Pasteur and the Institut Pasteur International Network.
Our core facility provides support in developing and characterizing VHH/nanobodies against the desired antigens. The main goal of this collaboration is to provide the right nanobodies that suit to the need of the user. We try not only to provide nanobodies but we can modify them: for example, according to the need, the nanobodies can be engineered to add a fluorochrome, to perform imaging; in this way, bispecific molecules can be created.
The CNCM is an International Depositary Authority (IDA) under the Budapest Treaty (BT). The CNCM receives deposits of accepts bacteria, filamentous fungi, yeasts, bacteriophages, viruses, animal, and human cell lines, for national and international patent procedures or other purposes requiring high secrecy requirements.
The provision of biological material preserved at the CNCM is governed by the BT implementing regulations.
The objective of the platform is to bridge biology and engineering in order to help the development of biomedical projects with a strong technological focus. Practically we provide the campus with essential technological bricks and expertise in microfluidics and biomaterials. We have developed in-house capabilities in the field of microfabrication, photo- and soft-lithography, advanced 3D cell culture systems, organoids and Organ-on-Chip technology especially targeted for the better understanding of complex cell-microenvironment interactions. In addition we also provide service based on standard organ-on-chip devices.