Metabolomics Core Facility
The main objective of the platform is to provide researchers with untargeted metabolomic strategies to help them answer their biological questions. The metabolomic team support researchers from the definition of experimental design to the interpretation of the results. They benefit from state-of-the-art instrumentation and computational methods. It includes the latest generation of high speed, high-resolution mass spectrometers as well as bioinformatics and statistical tools to analyze the complex high-dimensional data generated.
Our team is composed of experts in Mass Spectrometry (MS), metabolomics, lipidomics, computational and Mass Spectrometry Imaging (MSI)
The Cytometry Platform meets the needs of the scientific community for the multiparametric characterization of cell populations by providing high throughput cell analysis and sterile cell sorting services.
We provide flow cytometry services for analysis and sorting of cells, as well as training and expert consultation for project/experiment planning and collaborations.
Photonic BioImaging is a Unit of Technology and Service (UTechS) providing optical imaging expertise in life sciences and especially their application in studies on infectious biology.
Our activities include service rendering, training, technology-driven research and technology development. They are highly multi-disciplined, and collaborative, with the mission goal focused on the use of quantitative imaging and analysis to understand the processes of cell/tissue-biology, and their usurpation by infection and disease.
X-ray crystallography is the most widely used technique to reveal the 3D structure of biological macromolecules at atomic resolution. These structures are essential to understand the molecular details of protein function, protein-protein interactions and overall mechanistic reactions.
The crystallography core facility provides research groups working in the field of macromolecular crystallography with the expertise and technology required for high-throughput crystallization screening, X-ray diffraction measurements, and crystallographic computing.
We offer expertise in crystallography, from the crystallization of selected protein targets to the resolution of crystal structures of biological macromolecules. We ensure this mission by participating as a partner in scientific research projects collaborations involving studies of single proteins and protein complexes.
Protein samples submitted to our facility are subjected to high-throughput crystallization for X-ray studies, and depending on the size of the macromolecule, an aliquot of the sample can be used to prepare cryo-EM grids for imaging at the Institut Pasteur Nanoimaging facility. For X-ray data collection, the core facility is a member of the Block Allocation Group (BAG) of Institut Pasteur for regular access to synchrotrons ESRF (Grenoble) and SOLEIL (St Aubin).
We developed methods for detecting direct (binary) interactions between pairs of proteins, based on the complementation of luciferase enzymes (“split-luciferase”). We use these methods for high-throughput PPIs mapping in human cells (HEK293T). Our pipelines provide systematic, semi-quantitative and comparative PPIs maps. We are using the human ORFeome library, a collection of 17,000 human cDNAs, to gather pathway-dedicated sub-collections, i.e. an innate immunity-oriented library or a Ubiquitin Proteasome System library. The IRI laboratory focuses on virus/host PPIs, mainly studying respiratory and emerging viruses with the goal to identify molecular requisites of viral adaptation to human. We are open to collaborations for binary PPIs screening of our currently available libraries, for generating new human-proteome sub-libraries with any type of protein partners, should they be from a pathogen or not. Application of our PPIs screening pipeline to other organisms can be envisaged provided that orfeome of studied species are generated.
Our platform offers state-of the art large histotechnological laboratory with a specialized team. We are available for collaborative research. Many projects are currently ongoing on animal models of infectious diseases, cancer, immunotherapy or inflammation with internal or external research teams. Individuals wishing to develop collaborative research efforts are encouraged to contact us when developing research projects
Development and harmonization of innovative methods for comprehensive analysis of food-borne toxigenic bacteria, i.e. Staphylococci, Bacillus cereus and Clostridium perfringens.
150,000 samples of human origin accompanied by demographic (sex, age), clinical (pathology), preanalytical and biological (basic measurements) data.
Target-based (High Throughput Screening, HTS) and phenotypic (High Content Screening, HCS) screening assays are powerful tools to identify active chemical substances and genetic modulators of function (siRNA, miRNA, gRNA) providing starting points for drug design or for understanding biological processes. Using liquid handling devices, sensitive detectors and data processing software, HTS/HCS allow a researcher to rapidly test a large number of samples.
The Centre de Ressources Biologiques de l’Institut Pasteur, CRBIP, was created in 2001 as a transversal biobank infrastructure that includes microbial and human specimen collections of Institut Pasteur. The CRBIP receives, maintains, characterizes and supplies biological resources globally, in compliance with health and environmental safety standards and under applicable laws and regulations.
The Centre include 4 specific platforms:
-Collection of the Institut Pasteur (CIP)
-ICAReB-biobank : Integrated Collections for Adaptive Research in Biomedicine
-Collection Pasteur Culture of Cyanobacteria (PCC)
-National Collection of Cultures of Microorganisms
Access to biological resources distribution and deposits is only on remote basis:
-CRBIP catalogue of Microbial resources: https://catalogue-crbip.pasteur.fr/recherche_catalogue.xhtml. Contacts: cip@pasteur.fr (bacterial strains); collectionpcc@pasteur.fr (cyanobacterial strains); cvip@pasteur.fr (viral strains); cfip@pasteur.fr (fungal strains)
-Human biological resources: icareb-biobank@pasteur.fr
-Microbial strain and cell line deposits under the Budapest Treaty: cncm@pasteur.fr
Services: crbip-pmo@pasteur.fr
Current Prices (all prices are under revision)
These rates refer to 2023, please contact the team for a quote. Microbial resources:
-Bacteria and yeasts BSL1/2: 80 EUR
-Bacteria BSL3: 150 EUR -Strict anaerobic bacteria/Filamentous fungi: 100 EUR
-Cyanobacteria: 223 EUR
Human biological resources distribution: contact icareb-biobank@pasteur.fr
Microbial strains public deposits: free of charge
Microbial strain and cell line deposits under the Budapest Treaty (30 years deposits)
-Bacteria, Bacteriophages, Filamentous fungi, Yeasts: 701,27 EUR
-Virus propagated on cell lines: 1086,96 EUR
-Cell lines: 1448,27 EUR Services Microbial
-Preservation by freeze-drying: 162,60 EUR/strain
-Bacteria identification by MALDI-TOF MS: 74,50 EUR/strain
-Whole Genome Sequencing (Illumina) and taxonomic species classification: 391,40 EUR/strain
-Taxonomic species classification by MALDI-TOF MS + WGS: 460,50 EUR
-Bacterial genomic data assembly and analyses: 159,70 EUR/strain
-Sequence-based comparative genotyping: 319,40 EUR/strain
-Curation of data in existing cgMLST databases: 39,30 EUR
-Search of sequences of concern and genomic contextualization: 838,30 EUR
-Creation and/or curation of private genomic databases: inquire Human: see table below.