SANGER sequencing (capillary electrophoresis) / Genotyping / Fragment Analysis
Fiocruz’s Network Technological Platforms
The ABI 3730xl sequencer from Applied Biosystems is the highest throughput and cost-effective capillary electrophoresis equipment. Serving both sequencing and fragment analysis. It can sequence fragments of up to 800bp. The capillary sequencing platform offers four forms of services, where the user can choose to send samples to carry out the sequencing reaction procedure until samples are ready for running.
This platform is located in FIOCRUZ – Minas Gerais.
Fiocruz’s Network Technological Platforms.
Whole Exome Sequencing – S4 200 cycles; Genomic DNA extraction, library preparation and whole human exome sequencing on NovaSeq6000 platform (flow cell S4 – 200 cycles).
This platform is located in FIOCRUZ – Paraná.
Fiocruz’s Network Technological Platforms.
The services provided by the subunit include carrying out quantitative and qualitative assays, based on real-time fluorescence capture using Roche LightCycler 96 and Thermo QuantStudio5 equipments.
This platform is located in FIOCRUZ – Paraná.
Fiocruz’s Network Technological Platforms.
The sequencing platform supports the development of research projects that require nucleic acid sequencing technology, providing DNA sequencing services, fragment analysis and human leukocyte antigen (HLA). It contributes to the interpretation of results and also provides the following software for analysis: SeqScape, Sequence Scanner, BiodEdit and GeneMapper.
This platform is located in FIOCRUZ – Bahia.
Fiocruz’s Network Technological Platforms.
The Real-Time PCR Platform performs qualitative and quantitative assays based on real-time fluorescence capture for detection and/or estimation of the concentration of nucleic acids in the sample.
This platform is located in FIOCRUZ – Bahia.
Fiocruz’s Network Technological Platforms.
The main equipment are: MiSeq and MinION (NGS), QuantStudio5 (qPCR), Bioanalyzer (nucleic acid analysis) and Quibit (nucleic acid analysis). The platform also offers the use of KingFisher (automated nucleic acid extractor), Maxwell (automated nucleic acid extractor) and other accessory equipment necessary for the development of Molecular Biology techniques.
This platform is located in FIOCRUZ – Bahia.
Fiocruz’s Network Technological Platforms.
Bioinformatics Platform offers access to a high-performance computing (HPC) cluster featuring: Three computing nodes with a combined total of 768 CPUs; Two fat nodes equipped with a total of 8TB of RAM; Two GPU nodes equipped with 4 NVIDIA A100 GPUs; and SAS and SATA storage options. Our platform provides an array of bioinformatics programs accessible through environmental modules, and users can request the addition of new programs. Additionally, we offer access to popular databases, with the option to request additional databases as needed. Our infrastructure is purposefully designed for optimal performance in various computational tasks, including: Genomic and transcriptomic analysis; Protein modeling; Docking and dynamics simulations; Machine learning tasks. Feel free to utilize our resources for your bioinformatics research needs.
This platform is located in FIOCRUZ – Minas Gerais.
Fiocruz’s Network Technological Platforms.
Two types of sequencing technology.
The next-generation sequencing Platform RPT01O, located in Manaus/AM, at the Leonidas and Maria Deane Institute – Fiocruz Amazonia, provides the preparation and analysis of DNA/RNA libraries for various applications, such as genomic or amplicon sequencing, transcriptomics, and metagenomics. It has MiSeq equipment producing up to 15 Gb (gigabases) and 25 million sequences reads, as well as NextSeq, which can reach 120 Gb (gigabases) and 100 million sequences reads.
The Genomic Platform RPT01H is located at the Leonidas and Maria Deane Institute – Fiocruz Amazonia in Manaus/AM. Since 2011, it has had a technical manager and a coordinator. It has a genetic analyzer of the Sanger type, an ABI 3130 model from Applied Biosystems, which allows the analysis of sequences of genetic material fragments (PCR products and plasmids) of up to 96 reactions every 24 hours with a 4-channel capillary, generating between 500 to 900 base pairs. This platform is located in FIOCRUZ – Amazonas. Merge these two, its the same person, its just two types of sequencing tech. which can be specified under one platform
This platform is located in FIOCRUZ – Amazonas.
Fiocruz’s Network Technological Platforms.
The RPT-09-G subunit provides the equipment StepOnePlus™ and Quant Studio 5 (Thermofisher). Both thermocyclers with a detection system that uses LED lighting to detect four or six color channels respectively and are calibrated to operate with fluorophores: VIC®/ JOE™, ROX™, FAM™/SYBR® Green, TAMRA™/NED, or similar. In this way, the following tests can be carried out: SNP genotyping, gene expression analysis, microRNA expression, detection of genes, viral load analysis, and other absolute or relative quantitative assays. The following services are available:
a) Receipt of ready-made qPCR reactions using the TaqMan® methodology or similar fluorophores: VIC®/ JOE™, ROX™, FAM™/SYBR® Green, TAMRA™/NED or similar).
b) Receipt of ready-made qPCR reactions using the SYBR® Green methodology or similar.
c) Technical-scientific advice to assay design and interpretation
This platform is located in FIOCRUZ – Amazonas.
Fiocruz’s Network Technological Platforms.
The IGM Bioinformatics Platform was created in May 2012 and provides bioinformatics services to assist in research carried out at the institutional level. The services offered cover the creation, maintenance and mining of biological databases; analysis of RNA/DNA and protein sequences; training of personnel to use available bioinformatics resources and support for the development of new bioinformatics tools.
This platform is located in FIOCRUZ – Bahia.