Archive

Criterion: <span>Genomics & Transcriptomics - Sequencing & Analysis</span>

Support methods such as real-time PCR and SANGER sequencing. All members have RT-PCR technology.

Platform March 8, 2024

SANGER sequencing (capillary electrophoresis) / Genotyping / Fragment Analysis

Fiocruz’s Network Technological Platforms The ABI 3730xl sequencer from Applied Biosystems is the highest throughput and cost-effective capillary electrophoresis equipment. Serving both sequencing and fragment analysis. It can sequence fragments of up to 800bp. The capillary sequencing platform offers four forms of services, where the user can choose to send samples to carry out the […]

Platform March 8, 2024

Next Generation Sequencing Platform (Paraná)

Fiocruz’s Network Technological Platforms.Whole Exome Sequencing – S4 200 cycles; Genomic DNA extraction, library preparation and whole human exome sequencing on NovaSeq6000 platform (flow cell S4 – 200 cycles). This platform is located in FIOCRUZ – Paraná.

Platform March 8, 2024

RT-PCR (Paraná)

Fiocruz’s Network Technological Platforms.The services provided by the subunit include carrying out quantitative and qualitative assays, based on real-time fluorescence capture using Roche LightCycler 96 and Thermo QuantStudio5 equipments. This platform is located in FIOCRUZ – Paraná.

Platform March 8, 2024

SANGER sequencing (capillary electrophoresis) Platform (Bahia)

Fiocruz’s Network Technological Platforms. The sequencing platform supports the development of research projects that require nucleic acid sequencing technology, providing DNA sequencing services, fragment analysis and human leukocyte antigen (HLA). It contributes to the interpretation of results and also provides the following software for analysis: SeqScape, Sequence Scanner, BiodEdit and GeneMapper.This platform is located in […]

Platform March 8, 2024

RT-PCR (Bahia)

Fiocruz’s Network Technological Platforms. The Real-Time PCR Platform performs qualitative and quantitative assays based on real-time fluorescence capture for detection and/or estimation of the concentration of nucleic acids in the sample. This platform is located in FIOCRUZ – Bahia.

Platform March 8, 2024

Molecular Biology Platform (GNS, qPCR)

Fiocruz’s Network Technological Platforms.The main equipment are: MiSeq and MinION (NGS), QuantStudio5 (qPCR), Bioanalyzer (nucleic acid analysis) and Quibit (nucleic acid analysis). The platform also offers the use of KingFisher (automated nucleic acid extractor), Maxwell (automated nucleic acid extractor) and other accessory equipment necessary for the development of Molecular Biology techniques.This platform is located in […]

Platform March 8, 2024

Bioinformatics Platform

Fiocruz’s Network Technological Platforms.Bioinformatics Platform offers access to a high-performance computing (HPC) cluster featuring: Three computing nodes with a combined total of 768 CPUs; Two fat nodes equipped with a total of 8TB of RAM; Two GPU nodes equipped with 4 NVIDIA A100 GPUs; and SAS and SATA storage options. Our platform provides an array […]

Platform March 8, 2024

Next Generation Sequencing Platform (Amazonas)

Fiocruz’s Network Technological Platforms. Two types of sequencing technology.The next-generation sequencing Platform RPT01O, located in Manaus/AM, at the Leonidas and Maria Deane Institute – Fiocruz Amazonia, provides the preparation and analysis of DNA/RNA libraries for various applications, such as genomic or amplicon sequencing, transcriptomics, and metagenomics. It has MiSeq equipment producing up to 15 Gb […]

Platform March 8, 2024

RT-PCR Platform (Amazonas)

Fiocruz’s Network Technological Platforms. The RPT-09-G subunit provides the equipment StepOnePlus™ and Quant Studio 5 (Thermofisher). Both thermocyclers with a detection system that uses LED lighting to detect four or six color channels respectively and are calibrated to operate with fluorophores: VIC®/ JOE™, ROX™, FAM™/SYBR® Green, TAMRA™/NED, or similar. In this way, the following tests […]

Platform March 8, 2024

Bioinformatics Platform

Fiocruz’s Network Technological Platforms. The IGM Bioinformatics Platform was created in May 2012 and provides bioinformatics services to assist in research carried out at the institutional level. The services offered cover the creation, maintenance and mining of biological databases; analysis of RNA/DNA and protein sequences; training of personnel to use available bioinformatics resources and support […]