SANGER sequencing (capillary electrophoresis) Platform (Rio de Janeiro)

Fiocruz’s Network Technological Platforms.

The Genomic Platform RPT01A is located at the Oswaldo Cruz Institute – in Rio de Janeiro/RJ. It has a genetic analyzer of the Sanger type, an ABI 3730XL model from Applied Biosystems, which allows the analysis of sequences of genetic material fragments (PCR products and plasmids). The set of 50 cm capillaries (96-channel capillary) allows readings of up to 900 nucleotides (nt), with Q>20, and up to 1100 nt with Q>10, with runs of around 2h/plate. The equipment can be supplied with up to 16 plates. Samples must be sent in tubes or plates.

This platform is located in FIOCRUZ – Rio de Janeiro (IOC).