Criterion: <span>Flow Cytometry</span>

The CRB-IPM uses state-of-the-art equipment such as the Microflex Bruker MALDI-TOF (Matrix Assisted Laser Desorption Ionisation – Time of Flight) mass spectrometer and a flow cytometer (cell culture collections to come), as well as its skills in strain typing and bioinformatics to guarantee the identity and characteristics of the strains in the collection.

Fiocruz’s Network Technological Platforms. The Flow Cytometry Platform (RPT08I) offers immunophenotyping acquisition and analysis services; quantification of intracellular cytokines and cell supernatants, cultures, plasma and serum; cellular dermatology assays. The Platform currently has four cytometers, the CytoFLEX S (17 cores/19 parameters), Accuri C6 Plus (4 cores/6 parameters), FACSCelesta (12 cores/14 parameters) and FACSCan (5 cores/7 […]

Fiocruz’s Network Technological Platforms. The FACSymphony A1 anAnalyzing Cytometer can simultaneously detect 18 parameters, incluing cell size and complexity and 16 different fluorescence. It also has the ability to detect microparticle assays (90nm to 500nm). The equipment has four lasers: • Violet (405nm) Filters: 450/50, 525/50, 610/20, 670/30, 710/50 and 780/60 • Blue (488nm) Filters: […]

Fiocruz’s Network Technological Platforms.Performs FACS-based cell sorting, multiplex flow cytometry and single cell gene expression analysis. This platform is located in FIOCRUZ – Rio de Janeiro (IOC).

Fiocruz Network Facilities The Flow Cytometry facility offers multicolor cell analysis, including phenotype, cell viability, proliferation, cell cycle, cell activation and measurement of cytokines. FACSAria III is a digital flow cytometer and cell separator (sorting) with three lasers (ultraviolet -355 nm, blue – 488 nm, red – 633 nm). It has the capacity to analyze […]

Fiocruz’s Network Technological Platforms. The platform contains two analyzing cytometers and a cell sorter. The CytoFLEX LX (Beckman Coulter) has four lasers (405, 488, 561 and 638 nanometers) and the ability to analyze up to 18 parameters, including size and granularity. The BD FACSCanto II has three lasers (405, 488 and 633 nanometers) and the […]

Fiocruz’s Network Technological Platforms. The Flow Cytometry Platform has the following equipments: models FACSCalibur, LSRFortessa, FACAria II, FACSVerse, FACSymphony A5 (Becton Dickinson), Bioplex 200 (Merck and Bio-Rad) ImageStream MK II (Luminex) and Cytoflex S(Beckman Coulter) . They are used in a series of important procedures for carrying out diagnostic and monitoring methods for different pathologies […]

Fiocruz’s Network Technological Platforms. Equipment: FACSCanto™ II, with two lasers (blue – 488 nm, red – 633 nm), with capacity to analyze up to 8 parameters, including size and granularity. This platform is located in FIOCRUZ – Amazonas.

Fiocruz’s Network Technological Platforms.The Flow Cytometry Platform offers immunophenotyping data reading and analysis services; quantification of intracellular cytokines and culture supernatants, plasma and serum; cell proliferation and cell separation assays or “cell sorting”. The Platform currently has two cytometers, the FACSymphony S6 (19 colors/21 parameters) and the LSRFortessa (11 colors/13 parameters). The Platform also has […]

This platform offers advice and support to research groups or industry partners, using cell culture and flow cytometry as main technologies.