Fiocruz’s Network Technological Platforms.
The Fiocruz Rondônia Bioprospecting Platform offers 23 specialized services in the preparation, analysis, and purification of bioactives. Services include fractionation and isolation of biomolecules using different liquid chromatography techniques, characterization of the isoelectric point and determination of the molecular mass of protein samples, determination of the partial sequence of amino acid residues from the N-terminal region of purified proteins, and screening of in vitro molecular interactions between ligands of recombinant origin and analytes using the surface plasmon resonance technique, as well as determination of ligand/analyte interaction constants.

This platform is located in FIOCRUZ – Rondônia.

Fiocruz’s Network Technological Platforms.

The platform provides infrastructure and training on the following protein biophysical methods: protein production and purification, quantification, flourescent-based kinetics, protein interaction measurements, protein thermal, chemical and environmental stability and evaluation of protein secondary structure content.

This platform is located in FIOCRUZ – Pernambuco.

Fiocruz’s Network Technological Platforms

Analytical chemical platformAnalytical Chemical Methods – Chromatography, Mass Spectrometry, Nuclear Magnetic Ressonance, Infrared Spectrometry.

The Analytical chemical platform includes several different techniques, such as Nuclear Magnetic Resonance, Gas and Liquid Chromatography, Mass Spectrometry alone or hyphenated with chromatographic techniques and infrared spectroscopy. The platform also executes studies combining these techniques in order to help researchers develop analytical methods, validate methods, identify compounds and quantify compounds.

This platform is located in FIOCRUZ – Rio de Janeiro (FAR).

Fiocruz’s Network Technological Platforms.
The platform provides infrastructure and training in all stages of the process of determination and structural analysis of proteins: recombinant production, biophysical characterization, crystallization, structure determination by X-ray crystallography.

This platform is located in FIOCRUZ – Paraná.

Fiocruz’s Network Technological Platforms.

Rational design and in silico evolution of proteins (antibodies and fragments and therapeutic enzymes). Rational design of molecules for drug development.

This platform is located in FIOCRUZ – Ceará.

The Platform for the Characterization of Biological and Synthetic Nanovehicles (VBS) has applications primarily in active-ingredient delivery. Unique in Quebec, it works in close synergy with industry.
VBS provides a wide range of services to meet the needs of the university research community and healthcare, research, and biotechnology companies. Apart from the technical services it offers, VBS provides high-level training in bioimaging.

Located in Laval on the INRS – Armand-Frappier Santé Biotechnologie campus, the Electron Microscopy Platform (EM) has been offering you a range of high-quality imaging services since 1963. The platform makes it possible to carry out research using transmission and scanning electron microscopy technologies to meet the needs of universities, scientific communities and health, research or biotechnology industries. In addition to the technical services offered, the EMP allows the training of a high-level succession in life imaging.

The facility offers services to express recombinant proteins in prokaryotic and eukaryotic systems (E. coli, mammalian cells, insect cells); Optimization of the expression of soluble recombinant proteins; Refolding and soluble production of bacterial inclusion bodies; Maintenance of collections of expression vectors and bacterial strains.

The facility provides equipment, training, assistance, and technological innovations for determining and analyzing three-dimensional structures of proteins and macromolecular assemblies.

Our platform facilitates the exploration of protein-protein interactions (PPI) within living cells, utilizing a diverse range of high-throughput methods. We harness the power of the Bioluminescence Resonance Energy Transfer (BRET) technique for the precise quantification of proteins of interest, including, for example, pathogen/host protein pairs. What distinguishes our method is its capability to continuously monitor these interactions in real time within living cells for extended durations. This versatile approach supports a wide array of applications, including PPI validation, time-lapse PPI cell quantification, assessment of affinity in mutant PPI pairs, PPI competition studies, and screening for PPI modulators in drug discovery.

The Biotechnology Unit has extensive know-how on the production of soluble and membrane bound proteins in heterologous expression systems for structural and functional studies and is a member of the INSPIRED research infrastructure.

The currently available expression systems include bacterial, yeast, insect & mammalian cell systems. Large-scale production of proteins expressed in yeast and bacteria cells can be conducted in the fermenter unit.

Expressed recombinant proteins are purified through affinity, size exclusion or ion exchange chromatography, using FPLC systems.

The purified proteins can be subjected to crystallization in our crystallization robot. Data collection is carried out in synchrotron facilities, where HPI routinely acquires access and funding via the iNext-Discovery platform (https://inext-discovery.eu/). Structure determination and analysis is performed using standard crystallographic software (XDS, CCP4, Phenix, Coot etc.)