Criterion: <span>Macromolecular Science</span>

Nanoparticles, Recombinant Protein Production and Nanobodies.

Fiocruz’s Network Technological Platforms.The Fiocruz Rondônia Bioprospecting Platform offers 23 specialized services in the preparation, analysis, and purification of bioactives. Services include fractionation and isolation of biomolecules using different liquid chromatography techniques, characterization of the isoelectric point and determination of the molecular mass of protein samples, determination of the partial sequence of amino acid residues […]

Fiocruz’s Network Technological Platforms. The platform provides infrastructure and training on the following protein biophysical methods: protein production and purification, quantification, flourescent-based kinetics, protein interaction measurements, protein thermal, chemical and environmental stability and evaluation of protein secondary structure content. This platform is located in FIOCRUZ – Pernambuco.

Fiocruz’s Network Technological Platforms Analytical chemical platformAnalytical Chemical Methods – Chromatography, Mass Spectrometry, Nuclear Magnetic Ressonance, Infrared Spectrometry. The Analytical chemical platform includes several different techniques, such as Nuclear Magnetic Resonance, Gas and Liquid Chromatography, Mass Spectrometry alone or hyphenated with chromatographic techniques and infrared spectroscopy. The platform also executes studies combining these techniques in […]

Fiocruz’s Network Technological Platforms.The platform provides infrastructure and training in all stages of the process of determination and structural analysis of proteins: recombinant production, biophysical characterization, crystallization, structure determination by X-ray crystallography. This platform is located in FIOCRUZ – Paraná, Curitiba, Brazil. Head team: Beatriz Guimarães and Nilson Zanchin.

Fiocruz’s Network Technological Platforms. Rational design and in silico evolution of proteins (antibodies and fragments and therapeutic enzymes). Rational design of molecules for drug development. This platform is located in FIOCRUZ – Ceará.

The Platform for the Characterization of Biological and Synthetic Nanovehicles (VBS) has applications primarily in active-ingredient delivery. Unique in Quebec, it works in close synergy with industry. VBS provides a wide range of services to meet the needs of the university research community and healthcare, research, and biotechnology companies. Apart from the technical services it […]

The facility offers services to express recombinant proteins in prokaryotic and eukaryotic systems (E. coli, mammalian cells, insect cells); Optimization of the expression of soluble recombinant proteins; Refolding and soluble production of bacterial inclusion bodies; Maintenance of collections of expression vectors and bacterial strains.

The facility provides equipment, training, assistance, and technological innovations for determining and analyzing three-dimensional structures of proteins and macromolecular assemblies.

Our platform facilitates the exploration of protein-protein interactions (PPI) within living cells, utilizing a diverse range of high-throughput methods. We harness the power of the Bioluminescence Resonance Energy Transfer (BRET) technique for the precise quantification of proteins of interest, including, for example, pathogen/host protein pairs. What distinguishes our method is its capability to continuously monitor […]