Fiocruz’s Network Technological Platforms.

The Flow Cytometry Platform has the following equipments: models FACSCalibur, LSRFortessa, FACAria II, FACSVerse, FACSymphony A5 (Becton Dickinson), Bioplex 200 (Merck and Bio-Rad) ImageStream MK II (Luminex) and Cytoflex S(Beckman Coulter) . They are used in a series of important procedures for carrying out diagnostic and monitoring methods for different pathologies included in the panel of neglected diseases, in addition to being important for expanding the structure available for carrying out large-scale bioassays. The services provided by the Core Facility are: Immunophenotyping, high-resolution multiparametric analysis, data analysis (analysis in the FCAP program, FlowJo, Manager, Kaluza and IDEAS), Cell viability and proliferation, apoptosis and cell cycle, Analysis of cytokines/chemokines and other proteins, profile of immunoglobulins and microparticles and cell separation (sorting).

This platform is located in FIOCRUZ – Minas Gerais.