Language: <span>Portuguese</span>

Fiocruz’s Network Technological Platforms.The Fiocruz Rondônia Bioprospecting Platform offers 23 specialized services in the preparation, analysis, and purification of bioactives. Services include fractionation and isolation of biomolecules using different liquid chromatography techniques, characterization of the isoelectric point and determination of the molecular mass of protein samples, determination of the partial sequence of amino acid residues […]

Fiocruz’s Network Technological Platforms. The Flow Cytometry Platform (RPT08I) offers immunophenotyping acquisition and analysis services; quantification of intracellular cytokines and cell supernatants, cultures, plasma and serum; cellular dermatology assays. The Platform currently has four cytometers, the CytoFLEX S (17 cores/19 parameters), Accuri C6 Plus (4 cores/6 parameters), FACSCelesta (12 cores/14 parameters) and FACSCan (5 cores/7 […]

Fiocruz’s Network Technological Platforms. Fiocruz Rondônia’s Real Time PCR Platform has high-performance equipment and offers the entire scientific community the possibility of carrying out qualitative and/or quantitative reactions, in addition to evaluating gene expression. The platform also offers guidance on handling equipment and interpreting results with the aim of training qualified labor in the region. […]

Fiocruz’s Network Technological Platforms. The Seq Studio Genetic Analyzer capillary sequencer can sequence fragments of up to 800bp. The capillary sequencing platform offers the ready plate reading service, where the user sends their already sequenced and purified samples only for reading on the equipment (capillary electrophoresis), and also the complete sequencing reaction service, where the […]

Fiocruz’s Network Technological Platforms.The Fiocruz Rondônia Microscopy Platform features modern equipment for imaging materials or live biological samples, such as intravital, in vitro, or ex vivo microscopy with fresh or fixed and stained histological sections (including immunofluorescence). These can be analyzed using the available equipment: Nikon Eclipse Ti Confocal Microscope, Nikon Eclipse 80i Fluorescence Microscope, […]

Fiocruz’s Network Technological PlatformsIn vitro antiparasitic tests of natural compounds/extracts against promastigote forms of Leishmania spp. and asexual forms of Plasmodium falciparum W2. Cytotoxicity testing is also offered with cells of the J774 lineage (murine macrophages) and HepG2 (derived from a human hepatoma). All of these techniques are based on the use of fluorescence or […]

Fiocruz’s Network Technological Platforms. The FACSymphony A1 anAnalyzing Cytometer can simultaneously detect 18 parameters, incluing cell size and complexity and 16 different fluorescence. It also has the ability to detect microparticle assays (90nm to 500nm). The equipment has four lasers: • Violet (405nm) Filters: 450/50, 525/50, 610/20, 670/30, 710/50 and 780/60 • Blue (488nm) Filters: […]

Fiocruz’s Network Technological Platforms The Microscopy and Image Microanalysis Platform (RPT07E) of the Rene Rachou Institute/FIOCRUZ-Minas includes the following equipment in its infrastructure: an IVIS® Lumina XRMS Series III (Revvity), an Aperio Versa 8 slide scanner (Leica Biosystems), a C2+ Confocal Microscopy (Nikon), and an Axio Observer A.1 Fluorescence Microscopy (Zeiss). Since its implementation, the […]

Fiocruz’s Network Technological Platforms.Real-time PCR system, for Fast 96 and 384 wells plates. Performs presence and absence assays, absolute quantification, relative quantification and genotyping using allele-specific TaqMan probes. This platform is located in FIOCRUZ – Rio de Janeiro (IOC).

Fiocruz’s Network Technological Platforms. Real-time PCR system, for Fast 96, 384 wells plates and TaqMan Arrays (TLDA). Performs presence and absence assays, absolute quantification, relative quantification, genotyping using allele-specific TaqMan probes and High Resolution Melting (HRM). Performs TaqMan arrays, which are ideal for medium-to-high throughput validation and profiling studies in cancer, stem cells, inflammation, or […]