The Analytical Biochemistry and Proteomics Unit supports biomedical research projects based on mass spectrometry (MS) and proteomics. It also offers training, scientific assistance and access to EM-based proteomic technologies to the local scientific community; and contributes to local and regional education programs in this area.

The facility offers services to express recombinant proteins in prokaryotic and eukaryotic systems (E. coli, mammalian cells, insect cells); Optimization of the expression of soluble recombinant proteins; Refolding and soluble production of bacterial inclusion bodies; Maintenance of collections of expression vectors and bacterial strains.

The facility provides equipment, training, assistance, and technological innovations for determining and analyzing three-dimensional structures of proteins and macromolecular assemblies.

The Molecular Biology Unit (UBM) offers standardized nucleic acid sequencing services and fragment analysis by capillary electrophoresis. It also offers advice for the experimental design of genomic and transcriptomic studies through DNA microarrays or massive sequencing.

NGS and microarrays data analysis (Genome Assembly; Variant analysis; Transcriptomic and ribosomal profile analyses; Small RNAs data analysis; Sequence alignment and phylogenetic inference). Biostatistics and advice on specific software usage. Metagenomic data analyses and experimental design.

The Unit’s microscopy portfolio includes epifluorescence, confocal laser instruments, as well as “custom-made” multiphoton instruments. Our microscopes can perform experiments up to 5D (x / y / z / t / λ o τ). Among the tools that we master : FLIM, FRET, phasor graphs, autofluorescence, harmonic generation, deep tissue imaging, FCS and related techniques.

This platform offers advice and support to research groups or industry partners, using cell culture and flow cytometry as main technologies.

The unit provides high-level support in the field of animal genome modification (mice, rats and ruminants). We offer several techniques, such as pronuclear microinjection, homologous recombination in embryonic stem cells, lentiviral injection, transposons and the revolutionary CRISPR / Cas9 system. We also provide cryopreservation, in vitro fertilization and embryo rederivation in mice. Besides, the unit supplies mice and rats with high genetic and health status for researchers within the institute and the region.