Our expertise includes the folding of the synthesized proteins and the determination of disulfide bond patterns when present using proteomic tools (enzymatic digestion followed by mass spectrometry analysis). The secondary structure of the proteins can be studied using circular dichroism

The NMR laboratory is a technical platform of the Institut Pasteur de Lille. The laboratory is equipped with a 600MHz spectrometer with cryo-probe and sample changer, as well as a 300MHz spectrometer equipped with a sample changer.

The 300MHz low-field spectrometer is dedicated to the analytical control of small molecules to allow a fine analytical characterization of synthesized compounds or isolated natural products. Users can access it after having received training in its use

The Analysis Plateforme “Protéomiques et de Protéines Modifiées (P3M)” is a technical platform of the Institute Pasteur of Lille.

P3M offers expertise ranging from the identification of proteomes by nanoLC-MSMS and the use of databases or de novo analysis, to their quantification with or without labeling. The available equipment also allows the study of post-translational modifications (PTM) of small sizes with or without enrichment, as well as the characterization of protein maturation events.

P3M collaborates with PAGés platform for the specific study of glycosylations at the scale of sugars, glycopeptides and glycoproteins

ARIADNE is composed of two platforms: ARIADNE-Criblage, High-Content and High-throughput-Screening Platform and ARIADNE-ADME.

Working independently but complementary, their objective is to discover small chemical molecules useful in therapy or to explore the living, and to characterize their ADME properties. ARIADNE-Criblage brings together a set of expertise and equipment dedicated to the screening of biological disruptors such as small chemical molecules or siRNA libraries. ARIADNE-ADME allows to quantify the parameters which characterize the ability of molecules to be Absorbed, Distributed, Metabolized and Eliminated in vivo (ADME). Evaluation of ADME parameters early in the discovery process is widely used during target validation, hit-discovery, hit-to-lead optimisation stages

The Institut Pasteur de Lille-Biological Resources Center (IPL-CRB) is a technological platform of the Institut Pasteur de Lille created in 1998. Its missions are to collect, manage and enhance collections of biological resources, within the framework of projects concerning cardiovascular, metabolic, and neurodegenerative diseases.

the LIGAN-PM platform is dedicated to next-generation sequencing and other state-of-the-art genomic technologies. Our sequencing and genotyping systems are primarily dedicated to precision medicine. However, the platform is opened to all applications related to genetics and genomics regardless of species. This platform includes several sequencing systems (MiSeq, NextSeq, NovaSeq), various robots for library preparation, a platform dedicated to genotyping and other state-of-the-art systems of genetics and genomics (such as NanoString).

Go@L is the genomic plateforme of the UAR (Unité d’Appui et de Recherche) PLBS (Plateformes Lilloises en Biologie & Santé) UMS2014 US41. This plateforme is composed of 2 laboratories, one team (GFS) at the CHRU and one team (TAG) at the Institute Pasteur de Lille. The plateforme is specialized in genomic and transcriptomic based on the use and development of molecular biology applications using high throughput sequencing technologies.
Our main applications are :
– Genomic : WGS, WES, amplicon-seq, Tn-seq, CGH-arrays.
– Transcriptomic : RNA-seq, sRNA-seq, bacterial RNA-seq, NGS-RACE, RNA-seq full-length, DGE, single-cell RNA-seq, spatial transcriptomic.
– Metagenomic : targeted bacterial 16S metagenomic

The Animal Experimentation and High Technology Platform (PLEHTA) is specialized in hosting and establishing rodent animal models under highly controlled containment conditions. The platform includes multiple animal facilities (SPF, SOPF, biosafety levels 2 and 3), allowing maximum flexibility for the wide variety of demands by research groups from different disciplines including infections, cardiovascular and neurodegenerative diseases, metabolism, oncology, pharmacology and toxicology.

The BioImaging Center Lille (BICeL) is the academic platform for imaging, histology and cytometry of the University Hospital Campus, the Faculty of Science and Technology and the Pasteur Institute of Lille.

The BICeL brings together expertise, know-how and equipment on four platforms: (1) cytometry & flow imaging, (2) histology, (3) biophotonics and (4) electronic microscopy. Research & development in imaging techniques extends from the cell, to tissues and small rodent and aquatic organisms

The platform has transverse and diversified expertise. Its scientific scope includes in particular :
-Omics data analysis (genomics, transcriptomics, proteomics, metagenomics, epigenetics, …)
-Sequence annotation
-Integrative biology
-Systems biology
-Image data analysis
-High content screening data analysis
-Phylogeny
-Structural bioinformatics