The platform’s ambition is to develop serological or antigenic in vitro diagnostic test (IVD) as fast as possible, since 2020 for SARS-CoV-2, today for other viral, microbial or parasitic infectious agents, and tomorrow for global health applications on human, animal and environmental samples. The cross-disciplinary talents of our team weave together physics, chemistry, biology and engineering to imagine and design these diagnostic health tests of the future.

Mutualized Platform for Microbiology (P2M) is open to all the reference laboratories of the Institut Pasteur, in Paris and within the Pasteur Network, providing easy access to next generations multi-pathogenic sequencing (Illumina and Ion Torrent technologies).

The Platform was created in 2015 and has extensive experience of multipathogen sequencing.

Results can be downloadable remotly and sequence analysis can be carried out by the HUB bio info of the Institut Pasteur in Paris on request.

The objective of the platform is to bridge biology and engineering in order to help the development of biomedical projects with a strong technological focus. Practically we provide the campus with essential technological bricks and expertise in microfluidics and biomaterials. We have developed in-house capabilities in the field of microfabrication, photo- and soft-lithography, advanced 3D cell culture systems, organoids and Organ-on-Chip technology especially targeted for the better understanding of complex cell-microenvironment interactions. In addition we also provide service based on standard organ-on-chip devices.

The main objective of the platform is to provide researchers with untargeted metabolomic strategies to help them answer their biological questions. The metabolomic team support researchers from the definition of experimental design to the interpretation of the results. They benefit from state-of-the-art instrumentation and computational methods. It includes the latest generation of high speed, high-resolution mass spectrometers as well as bioinformatics and statistical tools to analyze the complex high-dimensional data generated.
Our team is composed of experts in Mass Spectrometry (MS), metabolomics, lipidomics, computational and Mass Spectrometry Imaging (MSI)

The Cytometry Platform meets the needs of the scientific community for the multiparametric characterization of cell populations by providing high throughput cell analysis and sterile cell sorting services.
We provide flow cytometry services for analysis and sorting of cells, as well as training and expert consultation for project/experiment planning and collaborations.

Photonic BioImaging is a Unit of Technology and Service (UTechS) providing optical imaging expertise in life sciences and especially their application in studies on infectious biology.

Our activities include service rendering, training, technology-driven research and technology development. They are highly multi-disciplined, and collaborative, with the mission goal focused on the use of quantitative imaging and analysis to understand the processes of cell/tissue-biology, and their usurpation by infection and disease.

The proteomics facility, headed by Mariette Matondo, is part of the Mass Spectrometry for Biology Utechs (MSBio) directed by Julia Chamot-Rooke. The overall objective of the platform is to develop innovative bottom-up proteomics strategies to address protein function, regulation and dynamic interactions, in particular in the context of infectious diseases. Our collaborators benefit from the expertise of the staff, as well as state-of-the-art instrumentation, including the latest generation of high speed, high-resolution mass spectrometers (Orbitrap), and bioinformatics tools. The proteomics analyses provided by the platform include:
• Identification of low abundant proteins in complex biological matrices
• Advanced quantitative proteomic strategies
• Characterization of post-translational modifications (PTMs)
• Analysis and dynamics of protein complexes
• Top-Down Proteomics to identify proteoforms.
• Coming soon : Single cell proteomics
Analyses can be performed either as research collaboration or service. Prior to starting the project, an experimental design is defined, and the staff provides advice on sample preparation. Results are further provided after a bioinformatic analysis of the data.

The nanoimaging core facility focusses on providing high-end cryo-electron microscopy services. We specialize in the three major workflows: single particle analysis (SPA), cryo-electron tomography/cryo focused ion-beam milling and correlative light and electron microscopy (cryo-FIB/cryo-CLEM) and microcrystal electron diffraction (MED). We help users collect their data but also organize many courses to train users to become fully autonomous. Our equipment is state-off-the art fully upgraded to the latest Thermofisher standards.